Mechanism of activation of the gastric aspartic proteinases: pepsinogen, progastricsin and prochymosin

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منابع مشابه

Mechanism of activation of the gastric aspartic proteinases: pepsinogen, progastricsin and prochymosin.

The gastric aspartic proteinases (pepsin A, pepsin B, gastricsin and chymosin) are synthesized in the gastric mucosa as inactive precursors, known as zymogens. The gastric zymogens each contain a prosegment (i.e. additional residues at the N-terminus of the active enzyme) that serves to stabilize the inactive form and prevent entry of the substrate to the active site. Upon ingestion of food, ea...

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Prochymosin activation by non-aspartic proteinases.

Prochymosin can be converted into chymosin by an action of external proteinases. Thus, thermolysin at pH 5.05 converts calf prochymosin into active Phe-chymosin, which is one amino acid longer than chymosin from the N-terminus with a yield of 73%. Even better results were achieved with prochymosin activation by Legionella pneumophila metalloproteinase. Apparently the stretch of prochymosin poly...

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The first step in the activation of chicken pepsinogen is similar to that of prochymosin.

Chicken pepsinogen was incubated at pH2.5 with pepstatin. The zymogen activated itself by a sequential mechanism and an intact peptide derived from residues 1-26 in the protein was released in the first step. This peptide was found to inhibit the milk-clotting activities of pig and chicken pepsins and calf chymosin but to different extents.

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1998

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj3350481